Newsletter

Announcements

• We welcome Dr. Minli Xing to BioNMR core facility. Minli is a protein NMR spectroscopist, her research interests include studying protein structure and function using NMR spectroscopy. Prior to joining the University of Michigan, Minli was the lab manager of the Biomolecular NMR Lab at the University of Kansas, where she operated and maintained a 600 MHz and an 800 MHz NMR spectrometer. During her work at the University of Kansas, she has developed extensive experience in protein structure determination, protein-protein and protein-ligand interaction, and STD-NMR fragment screening.

• NMR 600 (LSI) will be upgraded to Prodigy cryo-probe with NEO console in the coming year, stay tuned for updates.

Gateway NMR Meeting

This year University of Michigan will be hosting the Gateway NMR Meeting (details: https://sites.google.com/umich.edu/gatewaynmr2019) on Sep 21-22, 2019. Please try to attend this meeting to meet and learn about the exciting NMR research in the area.

NMR Seminars

• Dr. Charles Sanders from Vanderbilt School of Medicine Basic Sciences will talk about “Destabilization of the Human KCNQ1 Potassium Channel is the Most Common Cause of Long QT Syndrome Cardiac Arrhythmia” using NMR techniques on Sep 20th 2019 at 10AM in LSI library (details: https://www.lsi.umich.edu/events/2019-09/um-structure-seminar-destabilization-human-kcnq1-potassium-channel-most-common-cause-long-qt)
• Dr. Haribabu Arthanari from Harvard University & Dana-Farber Cancer Institute will be presenting a seminar titled ” Emerging methods in solution NMR and applications to illuminate blind spots in human biology” on Sep 20th 2019 at noon in 1400 Chem (details: https://lsa.umich.edu/biophysics/news-events/all-events.detail.html/64267-16274479.html)
• I will present a talk on advanced NMR techniques and newer methods to analyze NMR data on Oct 18th 2019 at 10AM in LSI library (details: https://www.lsi.umich.edu/events/2019-10/u-m-structure-seminar-debashish-sahu-phd)

Advanced Capabilities of NEO console on 800 Mhz NMR instrument

The NEO console has the capability of acquiring nested NMR experiments (combining up to five conventional NMR pulse sequences into one supersequence). We have working versions of all sequences mentioned in the latest paper Kupče Ē, Claridge TDW. New NOAH modules for structure elucidation at natural isotopic abundance (2019) J Magn Reson. 307 106568. (https://www.sciencedirect.com/science/article/pii/S1090780719301855?via%3Dihub)

NMR Technique Highlight: Accelerating 2D NMR relaxation dispersion experiments using iterated maps

Conventionally relaxation dispersion data would require collection of data using the full Nyquist grid. The authors had previously developed Fourier-based reconstruction method using iterated maps according to the Difference Map algorithm of Veit Elser (DiffMap), which has been further developed to exploit the strong correlations between 2D data slices in a pseudo-3D experiment. Details of the paper can be found in the link below:

Rovny, J., Blum, R.L., Loria, J.P., Barrett S,E. Accelerating 2D NMR relaxation dispersion experiments using iterated maps (2019) J Biomol NMR. https://doi.org/10.1007/s10858-019-00263-3

BioNMR core acknowledgement in scientific publications

A reminder to all users of BioNMR core facility users, please acknowledge the BioNMR core facility for all publications that use data collected in our facility.

Research reported in this publication was supported by the University of Michigan BioNMR Core Facility (U-M BioNMR). U-M BioNMR Core is grateful for support from U-M including the College of Literature, Sciences and Arts, Life Sciences Institute, College of Pharmacy and the Medical School along with the U-M Biosciences Initiative.

This helps us to keep track of our facility usage that leads to publications and justification for securing future funding for instrument upgrades.