- All NMR users need to use iLabs (https://bionmrcore.umich.edu/index.php/reservations/) to reserve time and use the instrument. Please note that providing short-codes directly to BioNMR core staff will not grant access to the instruments.
- NMR 400 (LSI) is currently down due to a sample breaking on the probe. Please use the NMR 600 (LSI) in the meantime.
- NMR 600CP (Chem) has issues with tuning to Nitrogen and the stability of the cryo-system causing issues for experiments longer than 2 hours. The NMR facility is in touch with Varian about this issue and will update the impacted users as things get sorted out.
NMR users of BioNMR core facility, please note the latest rates for instrument usage and consultation. The rates listed below are effective Nov 20th 2018.
|External Academic Rate|
|NMR 400 (LSI)||$11||$16.50||$33|
|NMR 600 (LSI)||$11||$16.50||$33|
|NMR 600 CP (Chem)||$9.50||$14.25||$28.50|
|NMR 800 (Chem)||$11.50||$17.25||$34.50|
|NMR Core Labor/|
Seminar on advanced metabolomics analysis on NMR data
Rafael Bruschweiler from Ohio State University is presenting his recent work where they employ advanced metabolomics tools on NMR data to study protein interactions. Dr. Bruschweiler is credited for the development of Covariance NMR, which reduces the NMR measurement time for multidimensional spectra of both solution and solid-state NMR. For more details on the seminar please see the flyer below.
Guide: Connecting remotely to NMR 800CP
As a convenience for the users of NMR 800CP, users can remotely monitor the progress of their experiments using VNC servers. Users have to manually start the X11VNC server and access using VNC viewers (like RealVNC viewer). Instructions on this process is available here: http://bionmrcore.umich.edu/wp-content/uploads/2018/11/Accessing-the-Bruker-800CP-machine-in-Chem-1047-using-VPN.pdf
NMR Technique Highlight: BEST and SOFAST NMR for assigning His & Tyr side-chains
In the following paper, the authors present a NMR technique that uses time-efficient resonance assignment of histidine and tyrosine side chains in uniformly 13C/15N-labeled proteins. The use of band-selective 13C pulses allows to deal with linear chains of coupled spins, thus avoiding signal loss that occurs in branched spin systems during coherence transfer. Details of the paper can be found in the link below:
Christou NE, Brutscher B, BEST and SOFAST experiments for resonance assignment of histidine and tyrosine side chains in 13C/15N labeled proteins (2018) J. Biomol NMR doi: 10.1007/s10858-018-0216-z Link: https://link.springer.com/article/10.1007/s10858-018-0216-z
NMR Webinars by Bruker
Bruker has various webinars on advanced NMR techniques and technologies.
Here are a few conferences (deadlines are fast approaching) that current BioNMR users will find useful:
- Biophysical Society 63nd Annual Meeting (March 2-6, 2019) at Baltimore, Maryland Link: http://www.biophysics.org/2019meeting
- PANIC 2019 Practical Applications of NMR in Industry Conference (March 3-7, 2019) at Hilton Head, South Carolina Link: http://www.panicnmr.com/
- 60th Experimental Nuclear Magnetic Resonance Conference (April 7 – 12, 2019) at Asilomar Conference Center, Pacific Grove, California
- Computational Aspects of Biomolecular NMR – Gordon Research Seminar (June 8-9, 2019) at Les Diablerets, Switzerland Link: https://www.grc.org/computational-aspects-of-biomolecular-nmr-grs-conference/2019/
- ISMAR 2019 21st International Society of Magnetic Resonance Conference joint with EUROMAR 2019 (August 25-30, 2019) at Berlin, Germany Link: http://www.ismar2019.org/
NMRFAM-Sparky tutorial videos
Sparky is the most popular NMR analysis tool for biomolecules for more than a decade. NMRFAM has taken over the original Sparky from UCSF for the continuous development to implement advances in biomolecular NMR field. This software is very useful for biological NMR community for NMR resonance assignment, structure prediction, semi-automatic structure calculation, etc. For details on the updated NMRFAM-Sparky software can be found here: https://nmrfam.wisc.edu/nmrfam-sparky-distribution/
There is a YouTube playlist of video tutorials on NMRFAM-Sparky software: