Protein Backbone Assignments using CCPNMR AnalysisAssign
Online workshop held on July 29th 2022
Presented by Dr. Minli Xing, Senior Lab Specialist of BioNMR Core Facility, University of Michigan.
Protein NMR
NMR data collection and chemical shift assignments for uniformly labeled well folded proteins (using Sparky)
Online workshop held on May 6th 2022
Presented by Debashish Sahu, Director of BioNMR core, University of Michigan
Collecting and analyzing protein backbone dynamics using T1/T2/NOE NMR based relaxation techniques
Online workshop held on December 8th 2021
Presented by Debashish Sahu, Director of BioNMR core, University of Michigan
Interactive graphs of Aliphatic Carbon and Proton Chemical shifts
In order to assist with chemical shift assignments of proteins, here are two plots of aliphatic chemical shifts based on BMRB data as of 10/17/2018.
For assignments of Cα and Cβ present in proteins, we usually run HNCACB and CBCA(CO)NH. Some residues such as Gly, Leu, Ala, Thr, Ser etc can be used as anchor points for starting assignments due to their unique distribution of aliphatic carbon chemical shift as shown below. Once can use this interactive map of chemical shifts below for chemical shift assignment.
A similar graph has been generated for assignments of aliphatic protons below. HCCH-TOCSY and H(CCCO)NH-TOCSY experiments give rise to chemical shifts of aliphatic protons, where this interactive distribution of chemical shift can be used for assignment purposes.