Big Secrets of Tiny Phenomena
We have been featured in the University of Michigan Literature Science and Arts News. Feel free to contact us if you have any NMR needs for your research projects.
We have been featured in the University of Michigan Literature Science and Arts News. Feel free to contact us if you have any NMR needs for your research projects.
This year University of Michigan will be hosting the Gateway NMR Meeting (details: https://sites.google.com/umich.edu/gatewaynmr2019) on Sep 21-22, 2019. Please try to attend this meeting to meet and learn about the exciting NMR research in the area.
The NEO console has the capability of acquiring nested NMR experiments (combining up to five conventional NMR pulse sequences into one supersequence). We have working versions of all sequences mentioned in the latest paper Kupče Ē, Claridge TDW. New NOAH modules for structure elucidation at natural isotopic abundance (2019) J Magn Reson. 307 106568. (https://www.sciencedirect.com/science/article/pii/S1090780719301855?via%3Dihub)
Conventionally relaxation dispersion data would require collection of data using the full Nyquist grid. The authors had previously developed Fourier-based reconstruction method using iterated maps according to the Difference Map algorithm of Veit Elser (DiffMap), which has been further developed to exploit the strong correlations between 2D data slices in a pseudo-3D experiment. Details of the paper can be found in the link below:
Rovny, J., Blum, R.L., Loria, J.P., Barrett S,E. Accelerating 2D NMR relaxation dispersion experiments using iterated maps (2019) J Biomol NMR. https://doi.org/10.1007/s10858-019-00263-3
A reminder to all users of BioNMR core facility users, please acknowledge the BioNMR core facility for all publications that use data collected in our facility.
Research reported in this publication was supported by the University of Michigan BioNMR Core Facility (U-M BioNMR). U-M BioNMR Core is grateful for support from U-M including the College of Literature, Sciences and Arts, Life Sciences Institute, College of Pharmacy and the Medical School along with the U-M Biosciences Initiative.
This helps us to keep track of our facility usage that leads to publications and justification for securing future funding for instrument upgrades.
Announcements
Recharge Rate
NMR users of BioNMR core facility, please note the latest rates for instrument usage and consultation. The rates listed below are effective Nov 20th 2018.
Instrument | U-M Rate ($/hr) | External Academic Rate ($/hr) | Corporate Rate ($/hr) |
NMR 400 (LSI) | $11 | $16.50 | $33 |
NMR 600 (LSI) | $11 | $16.50 | $33 |
NMR 600 CP (Chem) | $9.50 | $14.25 | $28.50 |
NMR 800 (Chem) | $11.50 | $17.25 | $34.50 |
NMR Core Labor/ Consultation | $61 | $91.50 | $183 |
Seminar on advanced metabolomics analysis on NMR data
Rafael Bruschweiler from Ohio State University is presenting his recent work where they employ advanced metabolomics tools on NMR data to study protein interactions. Dr. Bruschweiler is credited for the development of Covariance NMR, which reduces the NMR measurement time for multidimensional spectra of both solution and solid-state NMR. For more details on the seminar please see the flyer below.
Guide: Connecting remotely to NMR 800CP
As a convenience for the users of NMR 800CP, users can remotely monitor the progress of their experiments using VNC servers. Users have to manually start the X11VNC server and access using VNC viewers (like RealVNC viewer). Instructions on this process is available here: http://bionmrcore.umich.edu/wp-content/uploads/2018/11/Accessing-the-Bruker-800CP-machine-in-Chem-1047-using-VPN.pdf
NMR Technique Highlight: BEST and SOFAST NMR for assigning His & Tyr side-chains
In the following paper, the authors present a NMR technique that uses time-efficient resonance assignment of histidine and tyrosine side chains in uniformly 13C/15N-labeled proteins. The use of band-selective 13C pulses allows to deal with linear chains of coupled spins, thus avoiding signal loss that occurs in branched spin systems during coherence transfer. Details of the paper can be found in the link below:
Christou NE, Brutscher B, BEST and SOFAST experiments for resonance assignment of histidine and tyrosine side chains in 13C/15N labeled proteins (2018) J. Biomol NMR doi: 10.1007/s10858-018-0216-z Link: https://link.springer.com/article/10.1007/s10858-018-0216-z
NMR Webinars by Bruker
Bruker has various webinars on advanced NMR techniques and technologies.
Link: https://www.bruker.com/service/education-training/webinars/nmr-webinars.html
NMR Conferences
Here are a few conferences (deadlines are fast approaching) that current BioNMR users will find useful:
NMRFAM-Sparky tutorial videos
Sparky is the most popular NMR analysis tool for biomolecules for more than a decade. NMRFAM has taken over the original Sparky from UCSF for the continuous development to implement advances in biomolecular NMR field. This software is very useful for biological NMR community for NMR resonance assignment, structure prediction, semi-automatic structure calculation, etc. For details on the updated NMRFAM-Sparky software can be found here: https://nmrfam.wisc.edu/nmrfam-sparky-distribution/
There is a YouTube playlist of video tutorials on NMRFAM-Sparky software:
NMR Staff
Debashish Sahu, Ph. D.
Director, Biomolecular NMR Research Lab
LSA/LSI Core Facility
University of Michigan
Chemistry Building Room # 4015
930 N University Ave, Ann Arbor MI 48109
Email: debsahu@umich.edu
Phone: 734-647-3872
Wendy Feng, Ph. D.
Senior Lab Specialist
BioNMR Core Facility
University of Michigan
Life Sciences Institute # 3214
210 Washtenaw Ave, Ann Arbor MI 48109
Email: wendyfen@umich.edu
Phone: 734-615-5516
Emily Scott, Ph.D.
Professor, Medicinal Chemistry/Pharmacology/Biophysics
Faculty Director, BioNMR Core Lab
University of Michigan
428 Church Street
Ann Arbor, MI 48109-1065
Email: scottee@med.umich.edu
Phone: 734-764-3530
Lab webpage: http://scottlab.info
Cathy Andrews
Director of Operations
BioNMR Core Lab
University of Michigan
Life Sciences Institute # 3119
210 Washtenaw Ave, Ann Arbor MI 48109
Email: cathyand@umich.edu
Phone: 734-763-1200